Effect of altered dietary n-3 fatty acid intake upon plasma lipid fatty acid composition, conversion of [13C]α-linolenic acid to longer-chain fatty acids and partitioning towards β-oxidation in older men

被引:154
作者
Burdge, GC [1 ]
Finnegan, YE
Minihane, AM
Williams, CM
Wootton, SA
机构
[1] Univ Southampton, Inst Human Nutr, Southampton SO9 5NH, Hants, England
[2] Univ Reading, Hugh Sinclair Human Nutr Unit, Reading RG6 2AH, Berks, England
基金
英国生物技术与生命科学研究理事会;
关键词
alpha-linolenic acid; stable isotope; dietary n-3 polyunsaturated fatty acids; man;
D O I
10.1079/BJN2003901
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The effect of increased dietary intakes of alpha-linolenic acid (ALNA) or eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) for 2 months upon plasma lipid composition and capacity for conversion of ALNA to longer-chain metabolites was investigated in healthy men (52 (SD 12) years). After a 4-week baseline period when the subjects substituted a control spread, a test meal containing [U-C-13]ALNA (700 mg) was consumed to measure conversion to EPA, docosapentaenoic acid (DPA) and DHA over 48 h. Subjects were then randomised to one of three groups for 8 weeks before repeating the tracer study: (1) continued on same intake (control, n 5); (2) increased ALNA intake (10 g/d, n 4); (3) increased EPA+DHA intake (1.5 g/d, n 5). At baseline, apparent fractional conversion of labelled ALNA was: EPA 2.80, DPA 1.20 and DRA 0.04%. After 8 weeks on the control diet, plasma lipid composition and [C-13]ALNA conversion remained unchanged compared with baseline. The high-ALNA diet resulted in raised plasma triacylglycerol-EPA and -DPA concentrations and phosphatidylcholine-EPA concentration, whilst [C-13]ALNA conversion was similar to baseline. The high-(EPA+DHA) diet raised plasma phosphatidylcholine-EPA and -DHA concentrations, decreased [C-13]ALNA conversion to EPA (2-fold) and DPA (4-fold), whilst [C-13]ALNA conversion to DHA was unchanged. The dietary interventions did not alter partitioning of ALNA towards beta-oxidation. The present results indicate ALNA conversion was down-regulated by increased product (EPA+DHA) availability, but was not up-regulated by increased substrate (ALNA) consumption. This suggests regulation of ALNA conversion may limit the influence of variations in dietary n-3 fatty acid intake on plasma lipid compositions.
引用
收藏
页码:311 / 321
页数:11
相关论文
共 44 条
[31]   DIFFERENCES EXIST IN THE RELATIONSHIPS BETWEEN DIETARY LINOLEIC AND ALPHA-LINOLENIC ACIDS AND THEIR RESPECTIVE LONG-CHAIN METABOLITES [J].
MANTZIORIS, E ;
JAMES, MJ ;
GIBSON, RA ;
CLELAND, LG .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1995, 61 (02) :320-324
[32]   Myocardial membrane fatty acids and the antiarrhythmic actions of dietary fish oil in animal models [J].
McLennan, PL .
LIPIDS, 2001, 36 :S111-S114
[33]  
*MIN AGR FISH FOOD, 1997, 127 MAFF
[34]  
Mori TA, 2000, AM J CLIN NUTR, V71, P1085
[35]  
Pawlosky RJ, 2001, J LIPID RES, V42, P1257
[36]   In vivo conversion of linoleic acid to arachidonic acid in human adults [J].
Salem, N ;
Pawlosky, R ;
Wegher, B ;
Hibbeln, J .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1999, 60 (5-6) :407-410
[37]   THE INFLUENCE OF DIFFERENT TYPES OF OMEGA-3 POLY-UNSATURATED FATTY-ACIDS ON BLOOD-LIPIDS AND PLATELET-FUNCTION IN HEALTHY-VOLUNTEERS [J].
SANDERS, TAB ;
ROSHANAI, F .
CLINICAL SCIENCE, 1983, 64 (01) :91-99
[38]  
Shimokawa H, 2001, WORLD REV NUTR DIET, V88, P100
[40]  
Simopoulos Artemis P., 1999, Annals of Nutrition and Metabolism, V43, P127, DOI 10.1159/000012777