Porous poly(hydroxyethyl methacrylate) based monolith as a new adsorbent for affinity chromatography

被引:62
作者
Uzun, L
Say, R
Denizli, A [1 ]
机构
[1] Hacettepe Univ, Biochem Div, Dept Chem, TR-06532 Ankara, Turkey
[2] Anadolu Univ, Dept Chem, Eskisehir, Turkey
关键词
monoliths; antibody separation; protein purification; pseudospecific adsorbents; IgG;
D O I
10.1016/j.reactfunctpolym.2005.05.003
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A new approach was developed to obtain an efficient and cost effective purification of IgG from human plasma. Porous monoliths were obtained by the bulk polymerization of 2-hydroxyethyl methacrylate (HEMA) and N-methacryloyl(L)-histidinemethylester (MAH) conducted in a glass tube. Poly(HEMA-MAH) monolith had a specific surface area of 145.8 m(2)/g. Poly(HEMA-MAH) monolith was characterized by swelling studies, FTIR, scanning electron microscopy and elemental analysis. Poly(HEMA-MAH) monolith with a swelling ratio of 68%, and containing 47.8 mu mol MAH/g were used in the adsorption/desorption of IgG from aqueous solutions and human plasma. The maximum amount of IgG adsorption from aqueous solution in phosphate buffer was 9.8 mg/g at pH 7.4. A higher adsorption value was obtained from human plasma (up to 96.5 mg/g) with a purity of 95.3%. It was observed that IgG could be repeatedly adsorbed and desorbed with Poly(HEMA-MAH) monolith without significant loss in the adsorption capacity. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:93 / 102
页数:10
相关论文
共 41 条
[21]   CHROMATOGRAPHIC EVALUATION OF LARGE-PORE AND NON-POROUS POLYMERIC REVERSED PHASES [J].
LEE, DP .
JOURNAL OF CHROMATOGRAPHY, 1988, 443 :143-153
[22]   THE INTERACTION BETWEEN DIFFERENT DOMAINS OF STAPHYLOCOCCAL PROTEIN-A AND HUMAN POLYCLONAL IGG, IGA, IGM AND F(AB')2 - SEPARATION OF AFFINITY FROM SPECIFICITY [J].
LJUNGBERG, UK ;
JANSSON, B ;
NISS, U ;
NILSSON, R ;
SANDBERG, BEB ;
NILSSON, B .
MOLECULAR IMMUNOLOGY, 1993, 30 (14) :1279-1285
[23]   High-performance affinity chromatography with immobilization of protein A and L-histidine on molded monolith [J].
Luo, QZ ;
Zou, HF ;
Zhang, Q ;
Xiao, XZ ;
Ni, JY .
BIOTECHNOLOGY AND BIOENGINEERING, 2002, 80 (05) :481-489
[24]   Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) [J].
Luo, QZ ;
Zou, HF ;
Xiao, XZ ;
Guo, Z ;
Kong, L ;
Mao, XQ .
JOURNAL OF CHROMATOGRAPHY A, 2001, 926 (02) :255-264
[25]   Single-step purification of bispecific monoclonal antibodies for immunotherapeutic use by hydrophobic interaction chromatography [J].
Manzke, O ;
Tesch, H ;
Diehl, V ;
Bohlen, H .
JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 208 (01) :65-73
[26]   EFFICIENT RECOVERY OF RECOMBINANT PROTEINS USING MEMBRANE-BASED IMMUNOAFFINITY CHROMATOGRAPHY (MIC) [J].
NACHMAN, M ;
AZAD, ARM ;
BAILON, P .
BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (05) :564-571
[27]   Superporous agarose monoliths as mini-reactors in flow injection systems -: On-line monitoring of metabolites and intracellular enzymes in microbial cultivation processes [J].
Nandakumar, MP ;
Pålsson, E ;
Gustavsson, PE ;
Larsson, PO ;
Mattiasson, B .
BIOSEPARATION, 2000, 9 (04) :193-202
[28]  
Ostlund C, 1987, Dev Biol Stand, V66, P367
[29]   Effect of experimental conditions on strong biocomplimentary pairing in high-performance monolithic disk affinity chromatography [J].
Ostryanina, ND ;
Il'ina, OV ;
Tennikova, TB .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 770 (1-2) :35-43
[30]   A novel magnetic adsorbent for immunoglobulin-G purification in a magnetically stabilized fluidized bed [J].
Özkara, S ;
Akgöl, S ;
Çanak, Y ;
Denizli, A .
BIOTECHNOLOGY PROGRESS, 2004, 20 (04) :1169-1175