Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms

被引：18

作者：

Marti, R. ^{[2
,3
]}

Mieszkin, S. ^{[1
]}

Solecki, O. ^{[2
,3
]}

Pourcher, A. -M. ^{[2
,3
]}

Hervio-Heath, D. ^{[1
]}

Gourmelon, M. ^{[1
]}

机构：

[1] IFREMER, EMP, Lab Microbiol, F-29280 Plouzane, France

[2] Cemagref, UR GERE, Rennes, France

[3] Univ Europeenne Bretagne, Rennes, France

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2011年 / 111卷 / 05期

关键词：

Lactobacillus amylovorus; microbial source tracking; oxygen; persistence; pig-associated Bacteroidales markers; quantitative real-time PCR; river water; temperature; 16S RIBOSOMAL-RNA; REAL-TIME PCR; HUMAN FECAL POLLUTION; FRESH-WATER; ESCHERICHIA-COLI; MICROBIAL COMMUNITY; ANAEROBIC DIGESTER; HOST DIFFERENCES; WEANING PIGLETS; SP NOV;

D O I：

10.1111/j.1365-2672.2011.05131.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.

引用

页码：1159 / 1175

页数：17

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