Staurosporine induces apoptosis through both caspase-dependent and caspase-independent mechanisms

被引:364
作者
Belmokhtar, CA [1 ]
Hillion, J [1 ]
Ségal-Bendirdjian, E [1 ]
机构
[1] Hop St Louis, INSERM, U496, Ctr G Hayem, F-75010 Paris, France
关键词
staurosporine; apoptosis; caspase; L1210;
D O I
10.1038/sj.onc.1204436
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sensitivity of tumor cells to anticancer therapy depends on the ability of the drug to induce apoptosis, However, multiple signaling pathways control this induction and thus determine this sensitivity. We report here that staurosporine, a well known inducer of apoptosis in a wide range of cell lines, displays distinct ability to trigger apoptosis in two different L1210 sublines (termed 1,1210/ S and L1210/0). Staurosporine treatment resulted in an early cell death (within 3 h) in L1210/S cells, while in L1210/0 cells, death occurred only after 12 h. In both instances, death occurred by apoptosis. A broad spectrum caspase inhibitor, z-VAD-fmk, blocked early apoptosis in L1210/S cells but did not confer any protection on late apoptosis in L1210/0 cells. Protection by z-VAD-fmk observed in L1210/S cells was not lasting and unmasked a secondary process of cell death that also exhibited characteristics of apoptosis. Thus, staurosporine induces apoptotic cell death through at least two redundant parallel pathways. These two pathways normally coexist in L1210/S cells. However, the early cell death mechanism depending on caspase activation disguises the late caspase-independent apoptotic process. Staurosporine-induced apoptosis in L1210/0 cells develops only by the caspase-independent mechanism due to a general defect in caspase activation.
引用
收藏
页码:3354 / 3362
页数:9
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