Inhibitory effects of alkyl gallate and its derivatives on fatty acid desaturation

被引：19

作者：

Kawashima, H ^{[1
]}

Akimoto, K ^{[1
]}

Shirasaka, N ^{[1
]}

Shimizu, S ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT AGR CHEM,KYOTO 606,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1996年 / 1299卷 / 01期

关键词：

desaturase; polyunsaturated fatty acid; propyl gallate; microsome; (Mortierella); (liver);

D O I：

10.1016/0005-2760(95)00183-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alkyl gallate, which is known as an antioxidant, intensively inhibited Delta 5 and Delta 6 desaturation in both rat liver microsomes and an arachidonic acid-producing fungus Mortierella alpina 1S-4. The rat liver microsomal Delta 5 and Delta 6 desaturases were inhibited by gallic acid esterified with alcohols with various numbers of carbons, suggesting that the necessary structure in an esterified alcohol for the inhibition is not so strict. Among the three hydroxy groups in gallic acid, the m-hydroxy group was shown to be the necessary structure. Kinetic analyses revealed that propyl gallate is a noncompetitive inhibitor of Delta 5 desaturase (K-i = 2.6 . 10(-5) M) and Delta 6 desaturase (K-i = 1.7 . 10(-4) M). These data indicate that alkyl gallate is a new type of desaturase inhibitor and different from known natural. inhibitors, i.e., sesamin and curcumin.

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页码：34 / 38

页数：5

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