A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements

被引：17

作者：

Serrell, David B. ^{[1
,2
]}

Law, Jera ^{[1
]}

Slifka, Andrew J. ^{[1
]}

Mahajan, Roop L. ^{[3
]}

Finch, Dudley S. ^{[3
]}

机构：

[1] Natl Inst Stand & Technol, Boulder, CO 80305 USA

[2] Univ Colorado, Boulder, CO 80309 USA

[3] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA

来源：

BIOMEDICAL MICRODEVICES | 2008年 / 10卷 / 06期

关键词：

actin; bioMEMS; fibroblasts; micro-fabricated; single-cell;

D O I：

10.1007/s10544-008-9202-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.

引用

页码：883 / 889

页数：7

共 44 条

[1] Measurement techniques for cellular biomechanics in vitro [J].

Addae-Mensah, Kweku A. ;

Wikswo, John P. .

EXPERIMENTAL BIOLOGY AND MEDICINE, 2008, 233 (07) :792-809

[2] Vascular smooth muscle cell glycocalyx influences shear stress-mediated contractile response [J].

Ainslie, KM ;

Garanich, JS ;

Dull, RO ;

Tarbell, JM .

JOURNAL OF APPLIED PHYSIOLOGY, 2005, 98 (01) :242-249

[3] Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer [J].

Alenghat, FJ ;

Fabry, B ;

Tsai, KY ;

Goldmann, WH ;

Ingber, DE .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 277 (01) :93-99

[4] Measurement of local viscoelasticity and forces in living cells by magnetic tweezers [J].

Bausch, AR ;

Möller, W ;

Sackmann, E .

BIOPHYSICAL JOURNAL, 1999, 76 (01) :573-579

[5] Twisting integrin receptors increases endothelin-1 gene expression in endothelial cells [J].

Chen, JX ;

Fabry, B ;

Schiffrin, EL ;

Wang, N .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2001, 280 (06) :C1475-C1484

[6] Cellular control lies in the balance of forces [J].

Chicurel, ME ;

Chen, CS ;

Ingber, DE .

CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (02) :232-239

[7] Fast and slow dynamics of the cytoskeleton [J].

Deng, Linhong ;

Trepat, Xavier ;

Butler, James P. ;

Millet, Emil ;

Morgan, Kathleen G. ;

Weitz, David A. ;

Fredberg, Jeffrey J. .

NATURE MATERIALS, 2006, 5 (08) :636-640

[8] Engineering approaches to biomanipulation [J].

Desai, Jaydev P. ;

Pillarisetti, Anand ;

Brooks, Ari D. .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2007, 9 :35-53

[9] Creep function of a single living cell [J].

Desprat, N ;

Richert, A ;

Simeon, J ;

Asnacios, A .

BIOPHYSICAL JOURNAL, 2005, 88 (03) :2224-2233

[10] Physiological strains induce differentiation in human osteoblasts cultured on orthopaedic biomaterial [J].

Di Palma, F ;

Douet, M ;

Boachon, C ;

Guignandon, A ;

Peyroche, S ;

Forest, B ;

Alexandre, C ;

Chamson, A ;

Rattner, A .

BIOMATERIALS, 2003, 24 (18) :3139-3151

← 1 2 3 4 5 →