Immobilization of uricase upon polypyrrole-ferrocenium film

被引：22

作者：

Cete, Servet ^{[1
]}

Yasar, Ahmet ^{[1
]}

Arslan, Fatma ^{[1
]}

机构：

[1] Gazi Univ, Fac Arts & Sci, Dept Chem, Ankara, Turkey

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2007年 / 35卷 / 06期

关键词：

glutaraldehyde; immobilization; polypyrrole; uricase;

D O I：

10.1080/10731190701378634

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, uricase was immobilized by a glutaraldehyde/gelatine crosslinking procedure onto polypyrrole film. The K, value for immobilized enzyme 0.44mM was much higher than that of the free enzyme 0.39mM. V-max values were 8.4 x 10(-2) mM/dak and 7.1 x 10(-2) mM/dak for free and immobilized enzyme, respectively. The optimal pH values for free and immobilized enzymes were 8.5 and 8.0, respectively. The optimum temperature for both free and immobilized uricase was 35 degrees C and 55 degrees C. The enzyme activity after storage for 7 weeks was found to be 42% and 49% of the initial activity values for free and immobilized enzymes, respectively. The amperometric current obtained after 30 measurements at a constant uric acid concentration of 5.0 x 10(-5) M was found to be 77.7% of initial activity.

引用

页码：607 / 620

页数：14

共 38 条

[1] A biosensor based on urate oxidase-peroxidase coupled enzyme system for uric acid determination in urine [J].