Ultrasound-mediated stable transformation of potato tuber discs

被引：9

作者：

Sawahel, WA ^{[1
]}

机构：

[1] NATL RES CTR CAIRO,GENET ENGN & BIOTECHNOL DIV,CAIRO,EGYPT

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 11期

关键词：

D O I：

10.1007/BF00154665

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasmid DNA (pBARGUS), containing the selectable bar gene for resistance to the herbicide Basta, was delivered into potato tuber discs via ultrasonication. Transformed plants were identified by their ability to grow on a medium containing 1 mg phosphinothricin/l. Southern hybridization and plant resistance to the application of Basta indicated that a functional bar gene had integrated into potato chromosomal DNA.

引用

页码：821 / 824

页数：4

共 15 条

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