Bovine prion is endocytosed by human enterocytes via the 37 kDa/67 kDa laminin receptor

被引:88
作者
Morel, E
Andrieu, T
Casagrande, F
Gauczynski, S
Weiss, S
Grasso, J
Rousset, M
Dormont, D
Chambaz, J
机构
[1] UPMC, INSERM, UMR505, F-75006 Paris, France
[2] CEA, Serv Neurovirol, Fontenay Aux Roses, France
[3] CEA Saclay, Serv Pharmacol & Immunol, F-91191 Gif Sur Yvette, France
[4] Univ Munich, Mol Biol Lab, Genzentrum, Inst Biochem, D-80539 Munich, Germany
关键词
D O I
10.1016/S0002-9440(10)61192-3
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Some forms of transmissible spongiform encephalopathies result from oral infection. We have thus analyzed the early mechanisms that could account for an uptake of infectious prion particles by enterocytes, the major cell population of the intestinal epithelium. Human Caco-2/TC7 enterocytes cultured on microporous filters were incubated with different prion strains and contaminated brain homogenates in the apical compartment. internalization of infectious particles was analyzed by Western blotting and immunofluorescence. We observed internalization by enterocytes of prion particles from bovine spongiform encephalopathy brain homogenates but not from mouse-adapted scrapie-strain brain homogenates; or purified bovine spongiform encephalopathy scrapie-associated fibrils. Bovine prion particles were internalized via endocytosis within minutes of infection and were associated with subapical vesicular structures related to early endosomes. The endocytosis of the infectious bovine PrPSc was reduced by preincubating the cells with an anti-LRP/LR blocking antibody, identifying the 37 kDa/67 kDa laminin receptor (LRP/LR), which is apically expressed in Caco-2/TC7 cells, as the receptor for the infectious prion protein. Altogether, our results underscore a potential role of enterocytes; in the absorption of bovine prions during oral infection through specific LRP/LR-dependent endocytosis.
引用
收藏
页码:1033 / 1042
页数:10
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