Diagnostic Devices for Isothermal Nucleic Acid Amplification

被引：104

作者：

Chang, Chia-Chen ^{[1
]}

Chen, Chien-Cheng ^{[1
]}

Wei, Shih-Chung ^{[2
]}

Lu, Hui-Hsin ^{[1
]}

Liang, Yang-Hung ^{[1
]}

Lin, Chii-Wann ^{[1
,2
,3
]}

机构：

[1] Natl Taiwan Univ, Inst Biomed Engn, Taipei 10617, Taiwan

[2] Natl Taiwan Univ, Inst Biomed Elect & Bioinformat, Taipei 10617, Taiwan

[3] Natl Taiwan Univ, Ctr Emerging Mat & Adv Devices, Taipei 10617, Taiwan

来源：

SENSORS | 2012年 / 12卷 / 06期

关键词：

isothermal nucleic acid amplification; NASBA; SDA; RCA; LAMP; HDA; ROLLING-CIRCLE AMPLIFICATION; STRAND DISPLACEMENT AMPLIFICATION; HYBRIDIZATION CHAIN-REACTION; SEQUENCE-BASED AMPLIFICATION; REAL-TIME DETECTION; HEPATITIS-B-VIRUS; SENSITIVE DETECTION; DNA AMPLIFICATION; PROTEIN-DETECTION; MYCOBACTERIUM-TUBERCULOSIS;

D O I：

10.3390/s120608319

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.

引用

页码：8319 / 8337

页数：19

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