学术探索
学术期刊
新闻热点
数据分析
智能评审

LC-MS-based methodology for global metabolite profiling in metabonomics/metabolomics

被引：289
作者：
Theodoridis, Georgios
Gika, Helen G.
Wilson, Ian D. [1 ]
机构：
[1] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2008年 / 27卷 / 03期
关键词：
global metabolite profiling; metabolomics; metabonomics; metabotyping; separation; spectroscopic analysis;
D O I：
10.1016/j.trac.2008.01.008
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
in order to perform metabonomic investigations, it is necessary to generate comprehensive "global" metabolite profiles from complex samples, such as biofluids and tissues/tissue extracts. Analytical technologies based on liquid chromatography coupled to mass spectrometry (LC-MS) are destined to become a major source of global metabolite profiling data. Here, we describe and compare the utility of various analytical strategies and techniques currently used for LC-MS (capillary, high-performance and ultra-performance). We describe current challenges in the application of LC-MS-based techniques for metabonomic analysis. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：251 / 260
页数：10
相关论文
共 49 条
[11]   Metabolic fingerprinting of rat urine by LC/MS Part 1. Analysis by hydrophilic interaction liquid chromatography-electrospray ionization mass spectrometry [J].
Idborg, H ;
Zamani, L ;
Edlund, PO ;
Schuppe-Koistinen, I ;
Jacobsson, SP .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 828 (1-2) :9-13
[12]   Screening of biomarkers in rat urine using LC/electrospray ionization-MS and two-way data analysis [J].
Idborg-Björkman, H ;
Edlund, PO ;
Kvalheim, OM ;
Schuppe-Koistinen, I ;
Jacobsson, SP .
ANALYTICAL CHEMISTRY, 2003, 75 (18) :4784-4792
[13]   MZmine: toolbox for processing and visualization of mass spectrometry based molecular profile data [J].
Katajamaa, M ;
Miettinen, J ;
Oresic, M .
BIOINFORMATICS, 2006, 22 (05) :634-636
[14]   Data processing for mass spectrometry-based metabolomics [J].
Katajamaa, Mikko ;
Oresic, Matej .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1158 (1-2) :318-328
[15]   A comprehensive urinary metabolomic approach for identifying kidney cancer [J].
Kind, Tobias ;
Tolstikov, Vladimir ;
Fiehn, Oliver ;
Weiss, Robert H. .
ANALYTICAL BIOCHEMISTRY, 2007, 363 (02) :185-195
[16]   Metabolite profiling in rat urine by liquid chromatography/electrospray ion trap mass spectrometry. Application to the study of heavy metal toxicity [J].
Lafaye, A ;
Junot, C ;
Ramounet-Le Gall, B ;
Fritsch, P ;
Tabet, JC ;
Ezan, E .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (22) :2541-2549
[17]   The application of microbore UPLC/oa-TOF-MS and 1H NMR spectroscopy to the metabonomic analysis of rat urine following the intravenous administration of pravastatin [J].
Lenz, E. M. ;
Williams, R. E. ;
Sidaway, J. ;
Smith, B. W. ;
Plumb, R. S. ;
Johnson, K. A. ;
Rainville, P. ;
Shockcor, J. ;
Stumpf, C. L. ;
Granger, J. H. ;
Wilson, I. D. .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 44 (04) :845-852
[18]   Metabonomics with 1H-NMR spectroscopy and liquid chromatography-mass spectrometry applied to the investigation of metabolic changes caused by gentamicin-induced nephrotoxicity in the rat [J].
Lenz, EM ;
Bright, J ;
Knight, R ;
Westwood, FR ;
Davies, D ;
Major, H ;
Wilson, ID .
BIOMARKERS, 2005, 10 (2-3) :173-187
[19]   Cyclosporin A-induced changes in endogenous meta-bolites in rat urine:: a metabonomic investigation using high field 1H NMR spectroscopy, HPLC-TOF/MS and chemometrics [J].
Lenz, EM ;
Bright, J ;
Knight, R ;
Wilson, ID ;
Major, H .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2004, 35 (03) :599-608
[20]   A metabonomic investigation of the biochemical effects of mercuric chloride in the rat using 1H NMR and HPLC-TOF/MS:: time dependant changes in the urinary profile of endogenous metabolites as a result of nephrotoxicity [J].
Lenz, EM ;
Bright, J ;
Knight, R ;
Wilson, ID ;
Major, H .
ANALYST, 2004, 129 (06) :535-541
12345