LC-MS-based methodology for global metabolite profiling in metabonomics/metabolomics

被引：289

作者：

Theodoridis, Georgios

Gika, Helen G.

Wilson, Ian D. ^{[1
]}

机构：

[1] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2008年 / 27卷 / 03期

关键词：

global metabolite profiling; metabolomics; metabonomics; metabotyping; separation; spectroscopic analysis;

D O I：

10.1016/j.trac.2008.01.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

in order to perform metabonomic investigations, it is necessary to generate comprehensive "global" metabolite profiles from complex samples, such as biofluids and tissues/tissue extracts. Analytical technologies based on liquid chromatography coupled to mass spectrometry (LC-MS) are destined to become a major source of global metabolite profiling data. Here, we describe and compare the utility of various analytical strategies and techniques currently used for LC-MS (capillary, high-performance and ultra-performance). We describe current challenges in the application of LC-MS-based techniques for metabonomic analysis. (c) 2008 Elsevier Ltd. All rights reserved.

引用

收藏

页码：251 / 260

页数：10

相关论文

共 49 条

[31] UPLC/MSE;: a new approach for generating molecular fragment information for biomarker structure elucidation [J].

Plumb, Robert S. ;

Johnson, Kelly A. ;

Rainville, Paul ;

Smith, Brian W. ;

Wilson, Ian D. ;

Castro-Perez, Jose M. ;

Nicholson, Jeremy K. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (13) :1989-1994

[32] A rapid screening approach to metabonomics using UPLC and oa-TOF mass spectrometry: application to age, gender and diurnal variation in normal/Zucker obese rats and black, white and nude mice [J].

Plumb, RS ;

Granger, JH ;

Stumpf, CL ;

Johnson, KA ;

Smith, BW ;

Gaulitz, S ;

Wilson, ID ;

Castro-Perez, J .

ANALYST, 2005, 130 (06) :844-849

[33] Metabonomics: the use of electrospray mass spectrometry coupled to reversed-phase liquid chromatography shows potential for the screening of rat urine in drug development [J].

Plumb, RS ;

Stumpf, CL ;

Gorenstein, MV ;

Castro-Perez, JM ;

Dear, GJ ;

Anthony, M ;

Sweatman, BC ;

Connor, SC ;

Haselden, JN .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (20) :1991-1996

[34]

Robertson D.G., 2005, METABONOMICS TOXICIT

[35] Metabolomic identification of novel biomarkers of myocardial ischemia [J].

Sabatine, MS ;

Liu, E ;

Morrow, DA ;

Heller, E ;

McCarroll, R ;

Wiegand, R ;

Berriz, GF ;

Roth, FP ;

Gerszten, RE .

CIRCULATION, 2005, 112 (25) :3868-3875

[36] A pragmatic and readily implemented quality control strategy for HPLC-MS and GC-MS-based metabonomic analysis [J].

Sangster, Timothy ;

Major, Hilary ;

Plumb, Robert ;

Wilson, Amy J. ;

Wilson, Ian D. .

ANALYST, 2006, 131 (10) :1075-1078

[37] Automated 20 kpsi RPLC-MS and MS/MS with chromatographic peak capacities of 1000-1500 and capabilities in proteomics and metabolomics [J].

Shen, YF ;

Zhang, R ;

Moore, RJ ;

Kim, J ;

Metz, TO ;

Hixson, KK ;

Zhao, R ;

Livesay, EA ;

Udseth, HR ;

Smith, RD .

ANALYTICAL CHEMISTRY, 2005, 77 (10) :3090-3100

[38] XCMS: Processing mass spectrometry data for metabolite profiling using Nonlinear peak alignment, matching, and identification [J].

Smith, CA ;

Want, EJ ;

O'Maille, G ;

Abagyan, R ;

Siuzdak, G .

ANALYTICAL CHEMISTRY, 2006, 78 (03) :779-787

[39] Superheated water as an eluent for reversed-phase high-performance liquid chromatography [J].

Smith, RM ;

Burgess, RJ .

JOURNAL OF CHROMATOGRAPHY A, 1997, 785 (1-2) :49-55

[40] Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics [J].

Tolstikov, VV ;

Lommen, A ;

Nakanishi, K ;

Tanaka, N ;

Fiehn, O .

ANALYTICAL CHEMISTRY, 2003, 75 (23) :6737-6740

← 1 2 3 4 5 →