Expression of platelet-activating factor receptor transcript-1 but not transcript-2 by human bone marrow cells

被引：12

作者：

Desplat, V ^{[1
]}

Besse, A ^{[1
]}

Faucher, JL ^{[1
]}

Praloran, V ^{[1
]}

Denizot, Y ^{[1
]}

机构：

[1] Fac Med Limoges, Lab Hematol Expt, F-87025 Limoges, France

来源：

STEM CELLS | 1999年 / 17卷 / 02期

关键词：

bone marrow; CD34(+) progenitors; platelet-activating factor receptor; stromal cells; RT-PCR;

D O I：

10.1002/stem.170121

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The presence of platelet-activating factor receptor (PAF-R) transcripts 1 and 2 was investigated in human bone marrow cells by a reverse transcriptase polymerase chain reaction (RT-PCR) procedure mhich detected their simultaneous presence. RT-PCR experiments reveal PAF-R transcript 1 (but not 2) in freshly isolated mononuclear marrow cells, CD34(+) hematopoietic stem/progenitor cells and cultured marrow stromal cells. For these experiments, the 5637 human bladder carcinoma cell line is used as a positive control for the presence of PAF-R transcripts 1 and 2, Flow cytometry experiments confirm the presence of PAF-R on marrow stromal cells and CD34(+) stem/progenitor cells, In conclusion, the expression of PAF-R transcript 1, which mainly exists in circulating leukocytes, is also found in CD34(+) stem/progenitor cells and cells of the marrow microenvironment, strengthening the potential role of PAF during marrow hematopoiesis.

引用

页码：121 / 124

页数：4

共 15 条

[1]

Bellone G, 1997, BLOOD, V90, P21

[2] PAF AND HEMATOPOIESIS .3. PRESENCE AND METABOLISM OF PLATELET-ACTIVATING-FACTOR IN HUMAN BONE-MARROW [J].

DENIZOT, Y ;

TRIMOREAU, F ;

DUPUIS, F ;

VERGER, C ;

PRALORAN, V .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1995, 1265 (01) :55-60

[3]

Denizot Y, 1996, EXP HEMATOL, V24, P1327

[4] PAF and haematopoiesis .4. Platelet-activating factor increases DNA synthesis in human bone marrow cells [J].

Denizot, Y ;

Dupuis, F ;

Trimoreau, F ;

Verger, C ;

Allegraud, A ;

Praloran, V .

JOURNAL OF LIPID MEDIATORS AND CELL SIGNALLING, 1996, 15 (01) :1-4

[5]

DENIZOT Y, 1997, HDB EXPT PHARM PLATE, P483

[6] REGULATION OF HEMATOPOIESIS BY BONE-MARROW STROMAL CELLS AND THEIR PRODUCTS [J].

DORSHKIND, K .

ANNUAL REVIEW OF IMMUNOLOGY, 1990, 8 :111-137

[7] Production and metabolism of platelet-activating factor by human bone marrow cells [J].

Dupuis, F ;

Rougier, F ;

Trimoreau, F ;

Ostyn, E ;

Dulery, C ;

Praloran, V ;

Denizot, Y .

RESEARCH IN IMMUNOLOGY, 1997, 148 (02) :119-126

[8] Effect of platelet-activating factor on the growth of human erythroid and myeloid CD34+ progenitors [J].

Dupuis, F ;

Gachard, N ;

Allegraud, A ;

Dulery, C ;

Praloran, V ;

Denizot, Y .

MEDIATORS OF INFLAMMATION, 1998, 7 (02) :99-103

[9]

Fixe P, 1997, EUR CYTOKINE NETW, V8, P91

[10] CD34+ POSITIVE HEMATOPOIETIC-CELLS - BIOLOGY AND CLINICAL-APPLICATIONS [J].

HOLYOAKE, TL ;

ALCORN, MJ .

BLOOD REVIEWS, 1994, 8 (02) :113-124

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