Spectral-phase interference method for detecting biochemical reactions on a surface

被引:46
作者
Nikitin, PI
Gorshkov, BG
Valeiko, MV
Rogov, SI
机构
[1] Russian Acad Sci, Inst Gen Phys, Moscow 117769, Russia
[2] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117871, Russia
关键词
D O I
10.1070/QE2000v030n12ABEH001876
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
A simple optical method is proposed for the direct detection of biochemical reactions on a surface, which is insensitive to variations in the radiation intensity and refractive index of a solution. The method is based on the detection of the spectrum of reflected or transmitted radiation modulated by the interference in a sensitive layer of a large thickness (several tens and hundreds of microns), which can be a microscope cover glass with a deposited receptor layer. A change in the phase of the interference pattern in this spectrum is used as an information signal about a change in the thickness of the sensitive layer caused by a biochemical reaction. The method was tested in studies of the reactions of binding and detachment of proteins in real time. The root-mean-square noise of the method expressed in the layer thickness is 3 pm.
引用
收藏
页码:1099 / 1104
页数:6
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