Effects of MRI Contrast Agents on the Stem Cell Phenotype

被引:67
作者
Crabbe, Annelies [1 ]
Vandeputte, Caroline [2 ]
Dresselaers, Tom [3 ]
Ayuso Sacido, Angel [4 ]
Garcia Verdugo, Jose Manuel [4 ]
Eyckmans, Jeroen [5 ]
Luyten, Frank P. [5 ]
Van Laere, Koen [2 ]
Verfaillie, Catherine M. [1 ]
Himmelreich, Uwe [3 ]
机构
[1] Katholieke Univ Leuven, Stem Cell Inst, Louvain, Belgium
[2] Katholieke Univ Leuven, Div Nucl Med, Louvain, Belgium
[3] Katholieke Univ Leuven, Biomed NMR Unit, MOSAIC, Louvain, Belgium
[4] Univ Valencia, Ctr Invest Principe Felipe, CIBERNED, Valencia, Spain
[5] Katholieke Univ Leuven, Lab Skeletal Dev & Joint Disorders, Louvain, Belgium
关键词
Stem cells; Magnetic resonance imaging (MRI); Stroke; Ultrasmall superparamagnetic iron oxide [(U)SPIO] particles; Animal models; IRON-OXIDE PARTICLES; MARROW STROMAL CELLS; RAT T-CELLS; SELF-RENEWAL; DIFFERENTIATION; EXPRESSION; MIGRATION; TRACKING; CAPACITY; TRANSPLANTATION;
D O I
10.3727/096368910X494623
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The ultimate therapy for ischemic stroke is restoration of blood supply in the ischemic region and regeneration of lost neural cells. This might be achieved by transplanting cells that differentiate into vascular or neuronal cell types, or secrete trophic factors that enhance self-renewal, recruitment, long-term survival, and functional integration of endogenous stem/progenitor cells. Experimental stroke models have been developed to determine potential beneficial effect of stem/progenitor cell-based therapies. To follow the fate of grafted cells in vivo, a number of noninvasive imaging approaches have been developed. Magnetic resonance imaging (MRI) is a high-resolution, clinically relevant method allowing in vivo monitoring of cells labeled with contrast agents. In this study, labeling efficiency of three different stem cell populations [mouse embryonic stem cells (mESC), rat multipotent adult progenitor cells (rMAPC), and mouse mesenchymal stem cells (mMSC)] with three different (ultra)small superparamagnetic iron oxide [(U)SPIO] particles (Resovist (R), Endorem (R), Sinerem (R)) was compared. Labeling efficiency with Resovist (R) and Endorem (R) differed significantly between the different stem cells. Labeling with (U)SPIOs in the range that allows detection of cells by in vivo MRI did not affect differentiation of stem cells when labeled with concentrations of particles needed for MRI-based visualization. Finally, we demonstrated that labeled rMAPC could be detected in vivo and that labeling did not interfere with their migration. We conclude that successful use of (U)SPIOs for MRI-based visualization will require assessment of the optimal (U)SPIO for each individual (stem) cell population to ensure the most sensitive detection without associated toxicity.
引用
收藏
页码:919 / 936
页数:18
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