Isolation of a highly active PSII-LHCII supercomplex from thylakoid membranes by a direct method

被引：62

作者：

Eshaghi, S

Andersson, B

Barber, J ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, Wolfson Labs, London SW7 2AY, England

[2] Univ Stockholm, Dept Biochem, Arrhenius Labs Nat Sci, S-10691 Stockholm, Sweden

来源：

FEBS LETTERS | 1999年 / 446卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

oxygen evolution; photosystem II; thylakoid membrane;

D O I：

10.1016/S0014-5793(99)00149-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple and novel method to isolate a highly pure and active photosystem (PS) II complex, directly from thylakoid membranes. This complex is a discrete particle and contains all the proteins of the oxygen evolving complex and a set of chlorophyll nib binding proteins. The intactness of both the donor side and the acceptor side has resulted in a very high oxygen evolution activity and therefore offers a superior experimental system to that of PSII enriched membrane fragments in which there is heterogeneity in activities and biochemical composition. (C) 1999 Federation of European Biochemical Societies.

引用

页码：23 / 26

页数：4

共 17 条

[11] Specific association of photosystem II and light-harvesting complex II in partially solubilized photosystem II membranes [J].