Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for confirmation of AMPLICOR PCR-positive samples and comparison of its diagnostic performance according to storage conditions and preparation of endocervical specimens

被引:12
作者
Van Dyck, E [1 ]
Smet, H [1 ]
Van Damme, L [1 ]
Laga, M [1 ]
机构
[1] Inst Trop Med, Dept Microbiol, STD HIV Res & Intervent Unit, B-2000 Antwerp, Belgium
关键词
D O I
10.1128/JCM.39.6.2280-2282.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The AMPLICOR PCR was used to defect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in diluted phosphate-buffered saline, respectively.
引用
收藏
页码:2280 / 2282
页数:3
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