Identification of the cellular site of polychlorinated peptide biosynthesis in the marine sponge Dysidea (Lamellodysidea) herbacea and symbiotic cyanobacterium Oscillatoria spongeliae by CARD-FISH analysis

被引:89
作者
Flatt, P
Gautschi, J
Thacker, R
Musafija-Girt, M
Crews, P
Gerwick, W [1 ]
机构
[1] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA
[2] Univ Calif Santa Cruz, Dept Chem & Biochem, Santa Cruz, CA 95064 USA
[3] Univ Calif Santa Cruz, Inst Marine Sci, Santa Cruz, CA 95064 USA
[4] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
D O I
10.1007/s00227-005-1614-9
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Populations of the sponge Dysidea (Lamellodysidea) herbacea, which host the cyanobacterium Oscillatoria spongeliae, vary in their production of polychlorinated peptides. Peptide natural products previously isolated from D. herbacea are often halogenated and include dysidin, dysidinin, and a series of chlorinated diketopiperazines. Strikingly, the distinctive leucine-derived trichloromethyl signature of these compounds is shared only with metabolites of the marine cyanobacterium Lyngbya majuscula, and includes such compounds as barbamide and nordysidinin. Genetic information available for the barbamide biosynthetic gene cluster was used to successfully polymerase chain reaction (PCR) amplify a barB1 homolog (dysB1) from D. herbacea samples collected in Papua New Guinea. Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) analysis showed that dysB1 oligonucleotide probes hybridized to sequences in the filamentous cyanobacterial symbiont O. spongeliae. Consistent with this finding, a D. herbacea/O. spongeliae collection devoid of the polychlorinated peptides did not contain the barB1 homologs.
引用
收藏
页码:761 / 774
页数:14
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