Polyaniline based nucleic acid sensor

被引：62

作者：

Prabhakar, Nirmal ^{[1
,2
]}

Arora, Kavita ^{[1
]}

Singh, Harpal ^{[2
]}

Malhotra, Bansi D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Indian Inst Technol, Ctr Biomed Engn, New Delhi 110016, India

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2008年 / 112卷 / 15期

关键词：

D O I：

10.1021/jp711853q

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Twenty-bases long NH2-modified DNA and PNA probes specific to a pathogen (Mycobacterium tuberculosis) were covalently immobilized onto a polyaniline (PANI)/Au electrode to detect nucleic acid hybridization with complementary, one-base mismatch and noncomplementary targets within 30 s using Methylene Blue. The PNA-PANI/Au electrode exhibits improved specificity (1000 times) and detection limit (0.125 x 10(-18) M) as compared to that of the DNA-PANI/Au electrode (2.5 x 10(-18) M). These PNA-PANI/Au electrodes can be utilized for detection of hybridization with the complementary sequence in 5 min sonicated M. tuberculosis genomic DNA within I min of hybridization time. These DNA-PANI/Au and PNA-PANI/Au electrodes can be used 6-7 and 13-15 times, respectively.

引用

页码：4808 / 4816

页数：9

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