Interpretation of the cytostatic properties of sodium morpholyldithiocarbamate, a chelating agent

被引:12
作者
Cao, R
Fragoso, A
Villalonga, R
机构
来源
MONATSHEFTE FUR CHEMIE | 1996年 / 127卷 / 6-7期
关键词
dithiocarbamates; SOD inhibition; SOD mimic;
D O I
10.1007/BF00817269
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The antioxidant properties of sodium morpholyldithiocarbamate (MorDTC) were studied in order to contribute to the interpretation of its antitumor activity and synergetic effect over cis-platinum. MorDTC inhibits pyrogallol autooxidation better than vitamin C but does not dismutate the superoxide radical generated by the xanthine-xanthine oxidase system. Nevertheless, the complexes formed in situ between MorDTC and Mn(II), Co(II), Ni(II), and Cu(II) do dismutate the superoxide radical with a SOD-like activity (SOD = superoxide dismutase), expressed in terms of IC50 values within the range of 3.2 to 62 mu M. The highest activity corresponds to Mn(II) and Cu(II) complexes. The possible inhibitory action of MorDTC on erythrocyte SOD was also studied in vitro; however, the results were negative. Therefore, MorDTC should dismulate the superoxide radical after chelating metals without inhibiting SOD, the enzyme playing this role in vivo.
引用
收藏
页码:775 / 782
页数:8
相关论文
共 25 条
[1]  
ALLERBERGER F, 1989, MYCOSES, V32, P527, DOI 10.1111/j.1439-0507.1989.tb02176.x
[2]   CYTOTOXIC AND CYTOSTATIC ACTIVITY OF COPPER(II) COMPLEXES - IMPORTANCE OF THE SPECIATION FOR THE CORRECT INTERPRETATION OF THE INVITRO BIOLOGICAL RESULTS [J].
ARENA, G ;
BINDONI, M ;
CARDILE, V ;
MACCARRONE, G ;
RIELLO, MC ;
RIZZARELLI, E ;
SCIUTO, S .
JOURNAL OF INORGANIC BIOCHEMISTRY, 1993, 50 (01) :31-45
[3]   SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].
BEAUCHAM.C ;
FRIDOVIC.I .
ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&
[4]   ROLE OF AMINOACIDIC RESIDUES INSIDE ACTIVE-SITES OF METALLOPROTEINS [J].
BERTINI, I ;
LUCHINAT, C ;
MESSORI, L .
PURE AND APPLIED CHEMISTRY, 1988, 60 (08) :1261-1266
[5]  
BEYER W, 1971, MIKROCHIM ACTA, P610
[6]   DETERMINATION OF SUPEROXIDE DISMUTASE-LIKE ACTIVITY OF COPPER(II) COMPLEXES - RELEVANCE OF THE SPECIATION FOR THE CORRECT INTERPRETATION OF INVITRO O2- SCAVENGER ACTIVITY [J].
COSTANZO, LL ;
DEGUIDI, G ;
GIUFFRIDA, S ;
RIZZARELLI, E ;
VECCHIO, G .
JOURNAL OF INORGANIC BIOCHEMISTRY, 1993, 50 (04) :273-281
[7]  
DEALVARE LR, 1976, BIOCHEM BIOPH RES CO, V39, P687
[8]   Superoxide dismutase mimetic activity of the metal (II) complexes of a dithiocarbamate derivative of beta-cyclodextrin [J].
Fragoso, Alex ;
Cao, Roberto ;
Villalonga, Reynaldo .
JOURNAL OF CARBOHYDRATE CHEMISTRY, 1995, 14 (09) :1379-1386
[9]   ALKYL SUBSTITUENT EFFECTS IN DIAMAGNETIC DITHIOCARBAMATE COBALT(III) AND NICKEL(II) COMPLEXES [J].
HEALY, PC ;
CONNOR, JW ;
SKELTON, BW ;
WHITE, AH .
AUSTRALIAN JOURNAL OF CHEMISTRY, 1990, 43 (06) :1083-1095
[10]  
HEIKKILA RE, 1976, J BIOL CHEM, V251, P2182