Rapid Detection of Mycobacterium tuberculosis Complex and Rifampin Resistance in Smear-Negative Clinical Samples by Use of an Integrated Real-Time PCR Method

被引：118

作者：

Moure, Raquel ^{[1
]}

Munoz, Laura ^{[2
]}

Torres, Miriam ^{[1
]}

Santin, Miguel ^{[2
,4
]}

Martin, Rogelio ^{[1
,3
]}

Alcaide, Fernando ^{[1
,3
]}

机构：

[1] Bellvitge Hosp, IDIBELL, Dept Microbiol, Barcelona 08907, Spain

[2] Bellvitge Hosp, IDIBELL, Dept Infect Dis, Barcelona 08907, Spain

[3] Univ Barcelona, Dept Pathol & Expt Therapeut, Barcelona 08907, Spain

[4] Univ Barcelona, Dept Clin Sci, Barcelona 08907, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 03期

关键词：

PULMONARY TUBERCULOSIS; DIAGNOSIS; TESTS;

D O I：

10.1128/JCM.01831-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.

引用

页码：1137 / 1139

页数：3

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