Recruitment of HAT complexes by direct activator interactions with the ATM-related tra1 subunit

被引：292

作者：

Brown, CE

Howe, L

Sousa, K

Alley, SC

Carrozza, MJ

Tan, S

Workman, JL ^{[1
]}

机构：

[1] Penn State Univ, Howard Hughes Med Inst, Althouse Lab 306, University Pk, PA 16802 USA

[2] Penn State Univ, Dept Biochem & Mol Biol, Althouse Lab 306, University Pk, PA 16802 USA

[3] Penn State Univ, Dept Chem, Althouse Lab 306, University Pk, PA 16802 USA

来源：

SCIENCE | 2001年 / 292卷 / 5525期

关键词：

D O I：

10.1126/science.1060214

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Promoter-specific recruitment of histone acetyltransferase activity is often critical for transcriptional activation. We present a detailed study of the interaction between the histone acetyltransferase complexes SAGA and NuA4, and transcription activators. We demonstrate by affinity chromatography and photo-cross-linking Label transfer that acidic activators directly interact with Tra1p, a shared subunit of SAGA and NUA4. Mutations within the COOH-terminus of Tra1p disrupted its interaction with activators and resulted in gene-specific transcriptional defects that correlated with Lowered promoter-specific histone acetylation. These data demonstrate that the essential Tra1 protein serves as a common target for activators in both SAGA and NuA4 acetyltransferases.

引用

页码：2333 / 2337

页数：5