A simple and cost effective method for the quantification of 8-hydroxy-2′-deoxyguanosine from urine using liquid chromatography tandem mass spectrometry

被引：19

作者：

Crow, Brian ^{[1
]}

Bishop, Michael ^{[1
]}

Kovalcik, Kasey ^{[1
]}

Norton, Dean ^{[1
]}

George, Joe ^{[1
]}

Bralley, J. Alexander ^{[1
]}

机构：

[1] Metametrix Clin Lab, Duluth, GA 30096 USA

来源：

BIOMEDICAL CHROMATOGRAPHY | 2008年 / 22卷 / 04期

关键词：

8-hydroxy-2'-deoxyguanosine; DNA biomarkers; oxidative stress; LC-MS/MS;

D O I：

10.1002/bmc.946

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and high-throughput method for the determination of urinary levels of the oxidative stress biomarker, 8-hydroxy-2'-deoxyguanosine (8-OH-dG), has been developed and validated using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-MS/MS). The assay features a cheap and readily available non-isotopic internal standard, a single-step filtration sample preparation, and a total analysis time of 6 min including column re-equilibration. The method was validated based on linearity, accuracy (100-106%), precision (CV < 7%), sample preparation stability (<= 5%, 72 h). Intra-laboratory patient ranges were established comparing children and adults (n = 345). Copyright (c) 2007 John Wiley & Sons, Ltd.

引用

页码：394 / 401

页数：8

共 43 条

[1] DIETARY CARCINOGENS AND ANTICARCINOGENS - OXYGEN RADICALS AND DEGENERATIVE DISEASES [J].