The main auxin biosynthesis pathway in Arabidopsis

被引：746

作者：

Mashiguchi, Kiyoshi ^{[1
]}

Tanaka, Keita ^{[1
,2
]}

Sakai, Tatsuya ^{[3
]}

Sugawara, Satoko ^{[1
]}

Kawaide, Hiroshi ^{[2
]}

Natsume, Masahiro ^{[2
]}

Hanada, Atsushi ^{[1
]}

Yaeno, Takashi ^{[1
]}

Shirasu, Ken ^{[1
]}

Yao, Hong ^{[4
]}

McSteen, Paula ^{[4
]}

Zhao, Yunde ^{[5
]}

Hayashi, Ken-ichiro ^{[6
]}

Kamiya, Yuji ^{[1
]}

Kasahara, Hiroyuki ^{[1
]}

机构：

[1] RIKEN, Plant Sci Ctr, Kanagawa 2300045, Japan

[2] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Fuchu, Tokyo 1838509, Japan

[3] Niigata Univ, Grad Sch Sci & Technol, Niigata 9502181, Japan

[4] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA

[5] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

[6] Okayama Univ Sci, Dept Biochem, Okayama 7000005, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 45期

基金：

日本学术振兴会; 美国国家卫生研究院;

关键词：

plant hormone; metabolism; MOLYBDENUM COFACTOR SULFURASE; YUCCA FLAVIN MONOOXYGENASES; BOX PROTEIN TIR1; MOLECULAR-CLONING; REPRODUCTIVE DEVELOPMENT; ALDEHYDE OXIDASE; TRYPTOPHAN; GENE; ENCODES; THALIANA;

D O I：

10.1073/pnas.1108434108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indole-3-acetic acid (IAA) has been recognized as the major auxin for more than 70 y. Although several pathways have been proposed, how auxin is synthesized in plants is still unclear. Previous genetic and enzymatic studies demonstrated that both TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) and YUCCA (YUC) flavin monooxygenase-like proteins are required for biosynthesis of IAA during plant development, but these enzymes were placed in two independent pathways. In this article, we demonstrate that the TAA family produces indole-3-pyruvic acid (IPA) and the YUC family functions in the conversion of IPA to IAA in Arabidopsis (Arabidopsis thaliana) by a quantification method of IPA using liquid chromatography-electrospray ionization-tandem MS. We further show that YUC protein expressed in Escherichia coli directly converts IPA to IAA. Indole-3-acetaldehyde is probably not a precursor of IAA in the IPA pathway. Our results indicate that YUC proteins catalyze a rate-limiting step of the IPA pathway, which is the main IAA biosynthesis pathway in Arabidopsis.

引用

页码：18512 / 18517

页数：6

共 42 条

[1] The presence of CYP79 homologues in glucosinolate-producing plants shows evolutionary conservation of the enzymes in the conversion of amino acid to aldoxime in the biosynthesis of cyanogenic glucosides and glucosinolates [J].