Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins

被引：46

作者：

Cherezov, V ^{[1
]}

Caffrey, M ^{[1
]}

机构：

[1] Ohio State Univ, Columbus, OH 43210 USA

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2003年 / 36卷

关键词：

D O I：

10.1107/S002188980301906X

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple convenient and low- cost glass- based plate for high- throughput screening of membrane protein crystallization is described. The plates are robust and reduce dramatically the amount of protein and precipitant solution used per crystallization trial, while offering excellent optical properties for the detection of micro- crystals and crystals of colorless proteins. The plates were developed primarily for crystallization of membrane proteins in lipidic mesophases. They can also be used in batch crystallization of soluble and membrane proteins.

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页码：1372 / 1377

页数：6

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