MiR-107 suppresses cell proliferation and tube formation of Ewing sarcoma cells partly by targeting HIF-1β

被引：31

作者：

Chen, Jiajun ^{[1
]}

Zhou, Xin ^{[1
]}

Xiao, Qianren ^{[3
]}

Wang, Tengyu ^{[1
]}

Shao, Gaohai ^{[1
]}

Li, Yunyun ^{[2
]}

Zhang, Zhongzu ^{[1
]}

机构：

[1] Chongqing Med Univ, Yongchuan Hosp, Dept Orthoped, Chongqing 402160, Peoples R China

[2] Chongqing Med Univ, Yongchuan Hosp, Dept Gynecol & Obstet, Chongqing 402160, Peoples R China

[3] Nanchang Univ, Affiliated Hosp 1, Dept Orthoped, Nanchang 330006, Jiangxi, Peoples R China

来源：

HUMAN CELL | 2018年 / 31卷 / 01期

关键词：

Ewing's sarcoma; MiR-107; HIF-1; beta; Tube formation; LUNG-CANCER; TUMOR; HYPOXIA; PROGRESSION; PHENOTYPE;

D O I：

10.1007/s13577-017-0183-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

MicroRNAs serve a crucial role in the regulation of malignant biological behavior of Ewing's sarcoma (ES). Abnormal expression of miR-107 has been reported in a cohort of cancers, while its exact function in ES remains unclear. Hence, we explored the expression of miR-107 in ES cells and detected its effects on the malignant phenotype of ES cells. Firstly, we perceived the under-expression of miR-107 in human ES cells contrast with the human mesenchymal stem cells. Over-expression of miR-107 restrained cell proliferation and tube formation, arrested cell cycle progression, and facilitated cell apoptosis in SK-ES-1 and RD-ES cell lines. Furthermore, hypoxia inducible factor-1 beta (HIF-1 beta) was assumed as a target gene of miR-107. We confirmed the target role of HIF-1 beta in ES cells. Finally, restoring the expression of HIF-1 beta could partly abolish miR-107-mediated tumor suppression in ES cells. In conclusion, our results advised that miR-107 suppressed the malignant biological ability of ES cells through targeting HIF-1 beta.

引用

页码：42 / 49

页数：8

共 25 条

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Increased levels of serum and tissue miR-107 in human gastric cancer: Correlation with tumor hypoxia [J].