Separating stereoisomers of di-, tri-, and tetrapeptides using capillary electrophoresis with contactless conductivity detection

被引：28

作者：

Gong, Xiao Yang ^{[1
]}

Dobrunz, Dorninik ^{[1
]}

Kumin, Michael ^{[1
]}

Wiesner, Markus ^{[1
]}

Revell, Jefferson D. ^{[1
]}

Wennemers, Helma ^{[1
]}

Hauser, Peter C. ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4004 Basel, Switzerland

来源：

JOURNAL OF SEPARATION SCIENCE | 2008年 / 31卷 / 03期

关键词：

capillary electrophoresis; contactless conductivity detection; peptides; separation of stereoisomers;

D O I：

10.1002/jssc.200700461

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The separation and detection of small oligopeptides in CE with contactless conductivity detection were demonstrated. A strongly acidic separation buffer (0.5 M acetic acid) was employed in order to render the species cationic. Separation of the stereoisomers was achieved in typically 10-15 min by using either dimethyl-beta-CD (DM-beta-CD), (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C(6)H(4)), a combination of the two substances, or of histidine, as buffer additives. Calibration curves were determined for isomers of Gly-Asp and H-Pro-Asp-NH2, in the range of 0.05-0.5 mM and 0.1-1 mM, respectively, and were found to be linear. LODs were determined to be in the order of 1.0 mu M. The determination of isomeric impurities down to about 1% was found possible. Species showing good separation could also be successfully determined on an electrophoretic lab-on-chip device, with analysis times of a few minutes.

引用

页码：565 / 573

页数：9

共 72 条

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