Fluorescence imaging of cellular glutathione using a latent rhodamine

被引：192

作者：

Pires, Marcos M. ^{[1
]}

Chmielewski, Jean ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

ORGANIC LETTERS | 2008年 / 10卷 / 05期

关键词：

D O I：

10.1021/ol702769n

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Glutathione is a crucial component of the redox homeostasis of cells, and altered levels have been linked to human pathologies. We constructed a latent fluorophore (RhoSS) that responds to cellular thiols in vitro and in cyto following intracellular reduction by glutathione to yield rhodamine 110. Importantly, RhoSS was demonstrated to respond to changing levels of glutathione in cells. This compound represents a class of rationally designed latent fluorophores with exciting potential for monitoring cellular thiols.

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页码：837 / 840

页数：4

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