Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae

被引：42

作者：

Guaragnella, Nicoletta ^{[1
]}

Antonacci, Lucia ^{[1
,2
]}

Giannattasio, Sergio ^{[2
]}

Marra, Ersilia ^{[2
]}

Passarella, Salvatore ^{[1
]}

机构：

[1] Univ Molise, Dipartimento Sci Salute, I-86100 Campobasso, Italy

[2] CNR, Ist Biomembrane & Bioenerget, I-70126 Bari, Italy

来源：

FEBS LETTERS | 2008年 / 582卷 / 02期

关键词：

yeast; programmed cell death; acetic acid; hydrogen peroxide; catalase; superoxide dismutase;

D O I：

10.1016/j.febslet.2007.12.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zu-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H(2)O(2)) levels and enzyme activity as measured up to 200 min after AA treatment. AA-PCD does not occur in CTT1-Y, where H(2)O(2) levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H(2)O(2) levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：210 / 214

页数：5

共 25 条

[1] The apoptosis/necrosis transition in cerebellar granule cells depends on the mutual relationship of the antioxidant and the proteolytic systems which regulate ROS production and cytochrome c release en route to death [J].

Atlante, A ;

Bobba, A ;

Calissano, P ;

Passarella, S ;

Marra, E .

JOURNAL OF NEUROCHEMISTRY, 2003, 84 (05) :960-971

[2] Activity of plasma membrane H+-ATPase and expression of PMA1 and PMA2 genes in Saccharomyces cerevisiae cells grown at optimal and low pH [J].

Carmelo, V ;

Bogaerts, P ;

SaCorreia, I .

ARCHIVES OF MICROBIOLOGY, 1996, 166 (05) :315-320

[3] ONE-STEP TRANSFORMATION OF YEAST IN STATIONARY PHASE [J].

CHEN, DC ;

YANG, BC ;

KUO, TT .

CURRENT GENETICS, 1992, 21 (01) :83-84

[4] The copper chaperone for superoxide dismutase [J].

Culotta, VC ;

Klomp, LWJ ;

Strain, J ;

Casareno, RLB ;

Krems, B ;

Gitlin, JD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (38) :23469-23472

[5] Protective role of mitochondrial superoxide dismutase against high osmolarity, heat and metalloid stress in Saccharomyces cerevisiae [J].

Dziadkowiec, D. ;

Krasowska, A. ;

Liebner, A. ;

Sigler, K. .

FOLIA MICROBIOLOGICA, 2007, 52 (02) :120-126

[6] Redox control of apoptosis: An update [J].

Filomeni, Giuseppe ;

Ciriolo, Maria R. .

ANTIOXIDANTS & REDOX SIGNALING, 2006, 8 (11-12) :2187-2192

[7] Yeast apoptosis -: From genes to pathways [J].

Froehlich, Kai-Uwe ;

Fussi, Heike ;

Ruckenstuhl, Christoph .

SEMINARS IN CANCER BIOLOGY, 2007, 17 (02) :112-121

[8] Retrograde response to mitochondrial dysfunction is separable from TOR1/2 regulation of retrograde gene expression [J].

Giannattasio, S ;

Liu, ZC ;

Thornton, J ;

Butow, RA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (52) :42528-42535

[9] Acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-induced programmed cell death [J].

Giannattasio, S ;

Guaragnella, N ;

Corte-Real, M ;

Passarella, S ;

Marra, E .

GENE, 2005, 354 :93-98

[10] Hydrogen peroxide and superoxide anion production during acetic acid-induced yeast programmed cell death [J].

Guaragnella, N. ;

Antonacci, L. ;

Passarella, S. ;

Marra, E. ;

Giannattasio, S. .

FOLIA MICROBIOLOGICA, 2007, 52 (03) :237-240

← 1 2 3 →