Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

被引：17

作者：

Clements, Lauren R. ^{[1
,2
]}

Wang, Peng-Yuan ^{[3
]}

Harding, Frances ^{[1
]}

Tsai, Wei-Bor ^{[3
]}

Thissen, Helmut ^{[2
]}

Voelcker, Nicolas H. ^{[1
]}

机构：

[1] Flinders Univ S Australia, Sch Chem & Phys Sci, Bedford Pk, SA 5042, Australia

[2] CSIRO Mol & Hlth Technol, Melbourne, Vic 3168, Australia

[3] Natl Taiwan Univ, Dept Chem Engn, Taipei 10617, Taiwan

来源：

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE | 2011年 / 208卷 / 06期

基金：

澳大利亚研究理事会;

关键词：

biomaterial; electrochemistry; gradient surfaces; porous silicon; surface chemistry; RGD PEPTIDES; SURFACE; DIFFERENTIATION; TOPOGRAPHY;

D O I：

10.1002/pssa.201000320

中图分类号：

T [工业技术];

学科分类号：

120111 [工业工程];

摘要：

Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface. (C) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：1440 / 1445

页数：6

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