Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy

被引：1129

作者：

Neuman, Keir C. ^{[1
]}

Nagy, Attila ^{[2
]}

机构：

[1] NHLBI, Mol Biophys Lab, NIH, Bethesda, MD 20892 USA

[2] NHLBI, Lab Mol Physiol, NIH, Bethesda, MD 20892 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 06期

关键词：

D O I：

10.1038/NMETH.1218

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.

引用

页码：491 / 505

页数：15

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