DNA Immobilization and Detection on Cellulose Paper using a Surface Grown Cationic Polymer via ATRP

被引：42

作者：

Aied, Ahmed ^{[1
]}

Zheng, Yu ^{[1
]}

Pandit, Abhay ^{[1
]}

Wang, Wenxin ^{[1
]}

机构：

[1] Natl Univ Ireland, Network Excellence Funct Biomat, Galway, Ireland

来源：

ACS APPLIED MATERIALS & INTERFACES | 2012年 / 4卷 / 02期

基金：

爱尔兰科学基金会;

关键词：

atom transfer radical polymerization (ATRP); DNA sensors; fluorescence; cationic polymers; oligonucleotides; biomarkers; TRANSFER RADICAL POLYMERIZATION; SOLUBLE CONJUGATED POLYMERS; NUCLEIC-ACID PROBES; DOUBLE-STRANDED DNA; 2-(DIMETHYLAMINO)ETHYL METHACRYLATE; STAR POLYMERS; MICRORNA EXPRESSION; BRUSHES; HYBRIDIZATION; COPOLYMERS;

D O I：

10.1021/am201483h

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Cationic polymers with various structures have been widely investigated in the areas of medical diagnostics and molecular, biology because of their unique binding properties and capability to interact with biological molecules in complex biological environments.; In this work-we report the grafting of a linear cationic polymer from an atom transfer radical polymerization (ATRP) initiator bound to cellulose paper surface. We show successful binding of ATRP initiator onto cellulose paper and grafting of polymer chains from the immobilized initiator with ATRP. The cellulose paper grafted polymer was used in combination with PicoGreen (PG) to demonstrate detection of nucleic acids in the nanogram range in homogeneous solution and in a biological sample (serum). The results showed specific identification of hybridized DNA after addition of PG in both solutions.

引用

页码：826 / 831

页数：6

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