Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements

被引：14

作者：

Beach, L ^{[1
]}

Schweitzer, C ^{[1
]}

Scaiano, JC ^{[1
]}

机构：

[1] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2003年 / 1卷 / 03期

关键词：

D O I：

10.1039/b209284k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss : ds DNA ratio.

引用

页码：450 / 451

页数：2

共 8 条

[1]

Ausubel F. M., 1994, CURRENT PROTOCOLS MO

[2] Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye-DNA complexes [J].