An essential function of the C-elegans ortholog of TPX2 is to localize activated aurora A kinase to mitotic spindles

被引：94

作者：

Özlü, N

Srayko, M

Kinoshita, K

Habermann, B

O'Toole, ET

Müller-Reichert, T

Schmalz, N

Desai, A

Hyman, AA

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[2] Scion Comp Innovat, D-01307 Dresden, Germany

[3] Univ Calif San Diego, Dept Cellular & Mol Biol, Ludwig Inst Canc Res, La Jolla, CA 92093 USA

[4] Univ Colorado, Boulder Lab Electron Microscopy Cells 3D, Boulder, CO 80309 USA

来源：

DEVELOPMENTAL CELL | 2005年 / 9卷 / 02期

关键词：

D O I：

10.1016/j.devcel.2005.07.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In vertebrates, the microtubule binding protein TPX2 is required for meiotic and mitotic spindle assembly. TPX2 is also known to bind to and activate Aurora A kinase and target it to the spindle. However, the relationship between the TPX2-Aurora A interaction and the role of TPX2 in spindle assembly is unclear. Here, we identify TPXL-1, a C. elegans protein that is the first characterized invertebrate ortholog of TPX2. We demonstrate that an essential role of TPXL-1 during mitosis is to activate and target Aurora A to microtubules. Our data suggest that this targeting stabilizes microtubuies connecting kinetochores to the spindle poles. Thus, activation and targeting of Aurora A appears to be an ancient and conserved function of TPX2 that plays a central role in mitotic spindle assembly.

引用

页码：237 / 248

页数：12

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