Validation of ERIC PCR as a tool in epidemiologic research of Salmonella in slaughter pigs

被引:16
作者
Swanenburg, M [1 ]
Urlings, HAP [1 ]
Keuzenkamp, DA [1 ]
Snijders, JMA [1 ]
机构
[1] Univ Utrecht, Fac Vet Med, Dept Sci Food Anim Origin, NL-3508 TD Utrecht, Netherlands
关键词
Salmonella; pigs; ERIC PCR; epidemiology;
D O I
10.1038/sj.jim.2900568
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The purpose of this study was to test a protocol for a standardized ERIC PCR for its capability of genotyping Salmonella, isolated from pigs and their environment, in an epidemiologic approach. To test repeatability, four different Salmonella isolates were subjected to PCR three times. Furthermore, it was tested if the profiles on gel differed when a higher annealing temperature was used. Four Salmonella isolates were subjected to four different annealing temperatures (36, 40, 48 and 55 degrees C). Moreover it was tested if the differentiation of Salmonella isolates, based on the genotypes, differed when a higher annealing temperature was used. Eight Salmonella isolates were tested at normal (36 degrees C) and high (55 degrees C) annealing temperatures. The results showed that this standardized ERIC PCR protocol was an efficient tool for typing many Salmonella isolates within a short period of time. The profiles were repeatable within one PCR reaction, but some profiles differed when they were compared between reactions. A higher annealing temperature resulted in profiles that contained more or fewer bands. The differentiation between isolates, when comparing profiles, remained the same. It was concluded that the standardized ERIC PCR protocol is useful for genotyping Salmonella.
引用
收藏
页码:141 / 144
页数:4
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