A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences

被引：45

作者：

Blais, BW ^{[1
]}

Turner, G ^{[1
]}

Sooknanan, R ^{[1
]}

Malek, LT ^{[1
]}

机构：

[1] GENESCAPE,MISSISSAUGA,ON L4V 1T4,CANADA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 01期

关键词：

D O I：

10.1128/AEM.63.1.310-313.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (<10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.

引用

页码：310 / 313

页数：4

共 13 条

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