Monitoring dopamine release from single living vesicles with nanoelectrodes

被引:99
作者
Wu, WZ
Huang, WH
Wang, W
Wang, ZL
Cheng, JK [1 ]
Xu, T
Zhang, RY
Chen, Y
Liut, J
机构
[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
[2] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China
关键词
D O I
10.1021/ja050385r
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Carbon fiber nanoelectrodes (tip diameter = ca. 100 nm) have been first used to monitor real-time dopamine release from single living vesicles of single rat pheochromocytoma (PC12) cells. The experiments show that active and inactive release sites exist on the surface of cells, and the spatial distributions have been differentiated even in the same active release zone. It is first demonstrated that multiple vesicles can sequentially release dopamine at the same site of the cell surface, which possibly plays the main role in the dopamine release from PC12 cells. Copyright © 2005 American Chemical Society.
引用
收藏
页码:8914 / 8915
页数:2
相关论文
共 19 条
[11]   Extracellular pH is required for rapid release of insulin from Zn-insulin precipitates in beta-cell secretory vesicles during exocytosis [J].
Kennedy, RT ;
Lan, HA ;
Aspinwall, CA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (07) :1795-1796
[12]  
ROBERT DB, 2003, PHYSIOL REV, V83, P581
[13]   COLOCALIZATION OF CALCIUM-ENTRY AND EXOCYTOTIC RELEASE SITES IN ADRENAL CHROMAFFIN CELLS [J].
ROBINSON, IM ;
FINNEGAN, JM ;
MONCK, JR ;
WIGHTMAN, RM ;
FERNANDEZ, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (07) :2474-2478
[14]  
SCHROEDER TJ, 1994, J BIOL CHEM, V269, P17215
[15]   Dopamine neurons release transmitter via a flickering fusion pore [J].
Staal, RGW ;
Mosharov, EV ;
Sulzer, D .
NATURE NEUROSCIENCE, 2004, 7 (04) :341-346
[16]  
STANFORD JA, 1996, ANAL CHEM, V68, pA359
[17]   Transport, docking and exocytosis of single secretory granules in live chromaffin cells [J].
Steyer, JA ;
Horstmann, H ;
Almers, W .
NATURE, 1997, 388 (6641) :474-478
[18]   TEMPORALLY RESOLVED CATECHOLAMINE SPIKES CORRESPOND TO SINGLE VESICLE RELEASE FROM INDIVIDUAL CHROMAFFIN CELLS [J].
WIGHTMAN, RM ;
JANKOWSKI, JA ;
KENNEDY, RT ;
KAWAGOE, KT ;
SCHROEDER, TJ ;
LESZCZYSZYN, DJ ;
NEAR, JA ;
DILIBERTO, EJ ;
VIVEROS, OH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (23) :10754-10758
[19]   Transport, capture and exocytosis of single synaptic vesicles at active zones [J].
Zenisek, D ;
Steyer, JA ;
Almers, W .
NATURE, 2000, 406 (6798) :849-854