A multiple-capillary electrophoresis system for smallscale DNA sequencing and analysis

被引：61

作者：

Zhang, Jianzhong ^{[1
]}

Voss, Karl O. ^{[1
]}

Shaw, Diana F. ^{[1
]}

Roos, K. Pieter ^{[1
]}

Lewis, Darren F. ^{[1
]}

Yan, Juying ^{[1
]}

Jiang, Rong ^{[1
]}

Ren, Hongji ^{[1
]}

Hou, Joan Y. ^{[1
]}

Fang, Yu ^{[1
]}

Puyang, Xiaoling ^{[1
]}

Ahmadzadeh, Hossein ^{[1
]}

Dovichi, Norman J. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 24期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/27.24.e36

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A five-capillary system has been developed for DNA sequencing and analysis. The post-column fluorescence detector is based on a sheath-flow cuvette. The instrument provides uniform and continuous illumination of the samples. The cuvette virtually eliminates cross-talk in the fluorescence signal between capillaries. Discrete single-photon counting avalanche photodiodes provide high efficiency light detection. The instrument has detection limits (3 sigma) of 130 +/- 30 fluorescein molecules injected onto each capillary. Over 650 bases of sequence at 98.8% accuracy were generated in 100 min at 50 degrees C from M13mp18. Separation and detection of short tandem repeats proved efficient and accurate with the use of internal standards for direct comparison of migration times between capillaries.

引用

页数：7

共 30 条

[11]

HUNKAPILLER T, 1991, SCIENCE, V254, P67

[12] MULTIPLE-SHEATHFLOW CAPILLARY ARRAY DNA ANALYZER [J].

KAMBARA, H ;

TAKAHASHI, S .

NATURE, 1993, 361 (6412) :565-566

[13] MULTIWAVELENGTH FLUORESCENCE DETECTION FOR DNA SEQUENCING USING CAPILLARY ELECTROPHORESIS [J].

KARGER, AE ;

HARRIS, JM ;

GESTELAND, RF .

NUCLEIC ACIDS RESEARCH, 1991, 19 (18) :4955-4962

[14] ESTIMATION OF THE LIMIT OF DETECTION IN CHROMATOGRAPHY [J].

KNOLL, JE .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1985, 23 (09) :422-425

[15] OPTIMIZATION OF EXCITATION AND DETECTION GEOMETRY FOR MULTIPLEXED CAPILLARY ARRAY ELECTROPHORESIS OF DNA FRAGMENTS [J].

LU, XD ;

YEUNG, ES .

APPLIED SPECTROSCOPY, 1995, 49 (05) :605-609

[16] OPTIMIZATION OF ELECTRIC-FIELD STRENGTH FOR DNA-SEQUENCING IN CAPILLARY GEL-ELECTROPHORESIS [J].

LUCKEY, JA ;

SMITH, LM .

ANALYTICAL CHEMISTRY, 1993, 65 (20) :2841-2850

[17] CAPILLARY ARRAY ELECTROPHORESIS - AN APPROACH TO HIGH-SPEED, HIGH-THROUGHPUT DNA SEQUENCING [J].

MATHIES, RA ;

HUANG, XC .

NATURE, 1992, 359 (6391) :167-169

[18] Multiple capillary DNA sequencer that uses fiber-optic illumination and detection [J].

Quesada, MA ;

Zhang, SP .

ELECTROPHORESIS, 1996, 17 (12) :1841-1851

[19] SEPARATION OF DNA SEQUENCING FRAGMENTS AT 53 BASES MINUTE BY CAPILLARY GEL-ELECTROPHORESIS [J].

ROCHELEAU, MJ ;

DOVICHI, NJ .

JOURNAL OF MICROCOLUMN SEPARATIONS, 1992, 4 (05) :449-453

[20] FORMAMIDE MODIFIED POLYACRYLAMIDE GELS FOR DNA SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS [J].

ROCHELEAU, MJ ;

GREY, RJ ;

DA, YC ;

HARKE, HR ;

DOVICHI, NJ .

ELECTROPHORESIS, 1992, 13 (08) :484-486

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