共 35 条
Characterization of the mRNA capping apparatus of the microsporidian parasite Encephalitozoon cuniculi
被引:37
作者:

Hausmann, S
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机构: Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA

Vivarès, CP
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机构: Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA

Shuman, S
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机构:
Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA
机构:
[1] Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA
[2] Univ Clermont Ferrand, F-63177 Clermont Ferrand, France
关键词:
D O I:
10.1074/jbc.M109649200
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
A scheme of eukaryotic phylogeny has been suggested based on the structure and physical linkage of the enzymes that catalyze mRNA cap formation. Here we show that the intracellular parasite Encephatitozoon cuniculi encodes a complete mRNA capping apparatus consisting of separate triphosphatase (EcCet1), guanylyltransferase (EcCeg1), and methyltransferase (Ecm1) enzymes, which we characterize biochemically and genetically. The triphosphatase EcCetl belongs to a metal-dependent phosphohydrolase family that includes the triphosphatase components of the capping apparatus of fungi, DNA viruses, and the malaria parasite Plasmodium falciparum. These enzymes are structurally and mechanistically unrelated to the metal-independent cysteine phosphatase-type RNA tri-phosphatases found in metazoans and plants. Our findings support the proposed evolutionary connection between microsporidia and fungi, and they place fungi and protozoa in a common lineage distinct from that of metazoans and plants. RNA triphosphatase presents an attractive target for antiprotozoal/antifungal drug development.
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页码:96 / 103
页数:8
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