How cryo-EM is revolutionizing structural biology

被引：730

作者：

Bai, Xiao-Chen ^{[1
]}

McMullan, Greg ^{[1
]}

Scheres, Sjors H. W. ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 0QH, England

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2015年 / 40卷 / 01期

基金：

英国医学研究理事会;

关键词：

macromolecular complexes; single-particle analysis; cryo-electron microscopy; image processing; 3D reconstruction; electron detection; maximum-likelihood optimization; TRANSMISSION ELECTRON-MICROSCOPY; SINGLE-PARTICLE RECONSTRUCTION; LARGE RIBOSOMAL-SUBUNIT; ACTIVE PIXEL SENSOR; ATOMIC-RESOLUTION; 3-DIMENSIONAL RECONSTRUCTION; CRYOELECTRON MICROSCOPY; ANGSTROM RESOLUTION; ESCHERICHIA-COLI; PROTEIN CRYSTALS;

D O I：

10.1016/j.tibs.2014.10.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

For many years, structure determination of biological macromolecules by cryo-electron microscopy (cryo-EM) was limited to large complexes or low-resolution models. With recent advances in electron detection and image processing, the resolution by cryo-EM is now beginning to rival X-ray crystallography. A new generation of electron detectors record images with unprecedented quality, while new image-processing tools correct for sample movements and classify images according to different structural states. Combined, these advances yield density maps with sufficient detail to deduce the atomic structure for a range of specimens. Here, we review the recent advances and illustrate the exciting new opportunities that they offer to structural biology research.

引用

页码：49 / 57

页数：9

共 90 条

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