Presence of prostaglandin EP(4) receptor gene expression in a rat gastric mucosal cell line

被引:67
作者
Hassan, S
Kinoshita, Y
Min, D
Nakata, H
Kishi, K
Matsushima, Y
Asahara, M
Wang, HY
Okada, A
Maekawa, T
Matsui, H
Chiba, T
机构
[1] KOBE UNIV,SCH MED,DEPT MED,DIV GERONTOL,CHUO KU,KOBE 650,JAPAN
[2] UNIV TSUKUBA,INST CLIN MED,INST PHYS & CHEM SCI,CELL BANK,TSUKUBA,IBARAKI 305,JAPAN
关键词
RGM-1; EP(4); prostaglandin E(2); hexosamine;
D O I
10.1159/000201339
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
RGM-1 is an epithelial cell line established from gastric mucosa of adult Wistar rats. In this study, we characterized this newly established cell line by Northern blot analysis. We also investigated deoxyribonucleic acid (DNA) synthesis and hexosamine production in RGM-1 by PGE(2). Northern blot analysis did not detect any transcript of proton pump, gastrin receptor, histidine decarboxylase, somatostatin and pepsinogen 1, indicating the absence of characteristics of parietal, ECL, D and chief cells in RGM-1 cells. However, this periodic acid-Schiff (PAS)-positive cell line expressed prostaglandin EP(4) receptor mRNA but not EP(1) and EP(3) receptor mRNAs. [H-3]-thymidine incorporation into DNA of the cells was not increased by PGE(2). In contrast, PGE(2) increased hexosamine content in RGM-1 cells. These results suggest that RGM-1 may be a useful model of gastric mucosal cells and that PGE(2) plays a role on mucin synthesis in RGM-1 cells possibly via EP(4) receptors.
引用
收藏
页码:196 / 200
页数:5
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