Rapid conversion of unprotected galactose analogs to their UDP-derivatives for use in the chemo-enzymatic synthesis of unnatural oligosaccharides

被引：32

作者：

Uchiyama, T ^{[1
]}

Hindsgaul, O ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1998年 / 17卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1080/07328309808001892

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rapid conversion of D-galactose, its 2-deoxy, 3-deoxy, 4-deoxy and 6-deoxy derivatives and L-arabinose to their UDP-derivatives (2-7) is described. The procedure involves the in situ preparation of the per-O-trimethylsilylated glycopyranosyl iodides and their direct reaction with UDP. All six sugar nucleotides were active as substrates for beta(1-->4)-galactosyltransferase and were used to enzymatically prepare N-acetyllactosamine (8) and five of its analogs (9-13).

引用

页码：1181 / 1190

页数：10

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