Epidemiologic and diagnostic evaluation of a recent mumps outbreak using oral fluid samples

被引:16
作者
Reid, Fiona [1 ]
Hassan, Jaythoon [1 ]
Irwin, Fiona [1 ]
Waters, Allison [1 ,2 ]
Hall, William [1 ,2 ]
Connell, Jeff [1 ]
机构
[1] Univ Coll Dublin, Sch Med & Med Sci, Natl Virus Reference Lab, Dublin 4, Ireland
[2] Univ Coll Dublin, Sch Med & Med Sci, Ctr Res Infect Dis, Dublin 4, Ireland
关键词
mumps; genotype; IgM; vaccine failure;
D O I
10.1016/j.jcv.2007.10.009
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Objective: To determine the optimal strategy to investigate mumps virus infection in a partially vaccinated cohort. Study design: 122 oral fluid and serum samples were collected in a recent outbreak in Ireland. The largest age cohort, students aged 18-21 years old attending third level institutions, were investigated using virus isolation, detection of mumps specific IgM, IgG, RT-PCR and molecular genotyping. Results: 97% of patients had both detectable serum IgM and IgG. Mumps virus RNA was detected in 17 oral fluid samples and 14 of these originated from a single geographic location. Only 6 of the IgM positive samples had detectable mumps virus RNA whereas this could be detected in 11 IgM negative samples. Genotyping studies revealed that genotypes G and J were co-circulating during this outbreak. Conclusions: The use of an oral fluid sample to detect mumps virus RNA and IgM offers a major improvement over serological diagnosis in acute infection in both non-vaccinated or partially vaccinated individuals, and has the advantage that specimens are collected non-invasively. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:134 / 137
页数:4
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