The bone morphogenetic protein 15 gene is X-linked and expressed in oocytes

被引:410
作者
Dube, JL
Wang, P
Elvin, J
Lyons, KM
Celeste, AJ
Matzuk, MM
机构
[1] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA
[2] Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Human Mol Genet, Houston, TX 77030 USA
[4] Genet Inst, Dept Tissue Growth & Repair, Cambridge, MA 02140 USA
[5] Univ Calif Los Angeles, Sch Med, Dept Orthopaed Surg, Los Angeles, CA 90095 USA
[6] Univ Calif Los Angeles, Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA
关键词
D O I
10.1210/me.12.12.1809
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have taken advantage of the sequence relationships among the bone morphogenetic proteins (BMPs) to identify the mouse Bmp15 and human BMP15 genes. The 392-amino acid prepropeptides encoded by these BMP genes exhibit significant homology to each other, although the 70% identity observed between the 125-amino acid mature peptides is considerably lower than that seen in comparisons of other mouse and human orthologs. Both genes share a common structural organization and encode mature peptides that lack the cysteine residue normally involved in the formation of a covalent dimer. In addition, mouse Bmp15 and human BMP15 map to conserved syntenic regions on the X chromosome. We demonstrate, through a combination of Northern blot and in situ hybridization analyses, that mouse Bmp15 is expressed specifically in the oocyte beginning at the one-layer primary follicle stage and continuing through ovulation. Interestingly, BMP-15 is most closely related to and shares a coincident expression pattern with the mouse growth/differentiation factor 9 (GDF-9) gene that is essential for female fertility. Our findings will be important for defining the role of BMP-15 in follicular development.
引用
收藏
页码:1809 / 1817
页数:9
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