Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells

被引：3

作者：

Naganawa, Yasunori ^{[1
,2
]}

Shimmoto, Michie ^{[3
]}

Maleki, Soheila J. ^{[4
]}

Takase, Masayuki ^{[2
]}

Shinmoto, Hiroshi ^{[2
,5
]}

机构：

[1] Nagahama Inst Biosci & Technol, Shiga 5260829, Japan

[2] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[3] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1138613, Japan

[4] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA

[5] Tamagawa Univ, Machida, Tokyo 1948610, Japan

来源：

CYTOTECHNOLOGY | 2008年 / 57卷 / 02期

关键词：

antibody; epitope; epstein-barr virus; IgM; peanut allergen;

D O I：

10.1007/s10616-008-9142-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To analyze epitopes of peanut allergen Ara h1, Epstein-Barr virus-transformed human peripheral oligoclonal B-cells were cultured to obtain antibodies to Ara h1. The combined reaction pattern with six oligoclonal antibodies showed there were six antibody binding areas named a to f in Ara h1. We found the novel antibody binding area named "area c" (171-230aa).

引用

页码：177 / 180

页数：4

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