Self-assembly of the plant cell wall requires an extensin scaffold

被引:238
作者
Cannon, Maura C. [1 ]
Terneus, Kimberly [2 ]
Hall, Qi [1 ]
Tan, Li [2 ]
Wang, Yumei [1 ]
Wegenhart, Benjamin L. [2 ]
Chen, Liwei [2 ]
Lamport, Derek T. A. [3 ]
Chen, Yuning [2 ]
Kieliszewski, Marcia J. [2 ]
机构
[1] Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA
[2] Ohio Univ, Dept Chem & Biochem, Athens, OH 45701 USA
[3] Univ Sussex, Dept Biol & Environm Sci, Brighton BN1 9QG, E Sussex, England
关键词
cytokinesis; (hydroxy)proline-rich glycoprotein; nanotechnology;
D O I
10.1073/pnas.0711980105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cytokinesis partitions the cell by a cleavage furrow in animals but by a new cross wall in plants. How this new wall assembles at the molecular level and connects with the mother cell wall remains unclear. A lethal Arabidopsis embryogenesis mutant designated root-, shoot-, hypocotyl-defective (rsh) provides some clues: RSH encodes extensin AtEXT3, a structural glycoprotein located in the nascent cross wall or "cell plate" and also in mature cell walls. Here we report that electron micrographs of rsh mutant cells lacking RSH extensin correspond to a wall phenotype typified by incomplete cross wall assembly. Biochemical characterization of the purified RSH glycoprotein isolated from wild-type Arabidopsis cell cultures confirmed its identity as AtEXT3: a (hydroxy)proline-rich glycoprotein comprising 11 identical amphiphilic peptide repeats with a 28-residue periodicity: SOOOOKKHYVYKSOOOOVKHYSOOOVYH (O = Hyp), each repeat containing a hydrophobic isodityrosine cross-link motif (YVY, underlined). Atomic force microscopy of RSH glycoprotein imaged its propensity for self-assembly into a dendritic scaffold. Extensin peroxidase catalyzed in vitro formation of insoluble RSH gels with concomitant tyrosine cross-linking, hence this likelihood in muro. We conclude that self-assembling amphiphiles of lysine-rich RSH extensin form positively charged scaffolds in the cell plate. These react with negatively charged pectin to create an extensin pectate coacervate that may template further orderly deposition of the new cross wall at cytokinesis.
引用
收藏
页码:2226 / 2231
页数:6
相关论文
共 43 条
[1]   Di-isodityrosine, a novel tetrameric derivative of tyrosine in plant cell wall proteins: A new potential cross-link [J].
Brady, JD ;
Sadler, IH ;
Fry, SC .
BIOCHEMICAL JOURNAL, 1996, 315 :323-327
[2]   Pulcherosine, an oxidatively coupled trimer of tyrosine in plant cell walls: Its role in cross-link formation [J].
Brady, JD ;
Sadler, IH ;
Fry, SC .
PHYTOCHEMISTRY, 1998, 47 (03) :349-353
[3]   Nature designs tough collagen: Explaining the nanostructure of collagen fibrils [J].
Buehler, Markus J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (33) :12285-12290
[4]   Collagen-related peptides: Self-assembly of short, single strands into a functional biomaterial of micrometer scale [J].
Cejas, Mabel A. ;
Kinney, William A. ;
Chen, Cailin ;
Leo, Gregory C. ;
Tounge, Brett A. ;
Vinter, Jeremy G. ;
Joshi, Pratik P. ;
Maryanoff, Bruce E. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (08) :2202-+
[5]  
CHANG SS, 1994, PLANT J, V5, P551, DOI 10.1046/j.1365-313X.1994.5040551.x
[6]   Cantilever dynamics and quality factor control in AC mode AFM height measurements [J].
Chen, Liwei ;
Yu, Xuechun ;
Wang, Dan .
ULTRAMICROSCOPY, 2007, 107 (4-5) :275-280
[7]   A POSSIBLE ROLE OF HYDROXYPROLINE-CONTAINING PROTEINS IN CESSATION OF CELL ELONGATION [J].
CLELAND, R ;
KARLSNES, AM .
PLANT PHYSIOLOGY, 1967, 42 (05) :669-&
[8]   AN INTRAMOLECULAR LINKAGE INVOLVING ISODITYROSINE IN EXTENSIN [J].
EPSTEIN, L ;
LAMPORT, DTA .
PHYTOCHEMISTRY, 1984, 23 (06) :1241-1246
[10]   The cell wall hydroxyproline-rich glycoprotein RSH is essential for normal embryo development in Arabidopsis [J].
Hall, Q ;
Cannon, MC .
PLANT CELL, 2002, 14 (05) :1161-1172