Binding of a native titin fragment to actin is regulated by PIP2

Titin is a giant protein which extends from Z-line to M-line in striated muscles. We report here the purification of a 150-kDa titin fragment, obtained after V8 protease treatment of myofibrils, This polypeptide was located at the N1-line level, in a titin part known to exhibit stiff properties correlated to an association with actin, By solid or liquid phase binding assays and cosedimentation, we have clearly demonstrated a direct, saturable and relative high affinity binding of the native titin fragment to F-actin, The 150-kDa titin fragment was also shown to accelerate actin polymerization. Furthermore, the actin-titin interaction was found to be inhibited by phosphoinositides. (C) 1998 Federation of European Biochemical Societies.