Analysis of fentanyl and norfentanyl in human plasma by liquid chromatography-tandem mass spectrometry using electrospray ionization

被引：37

作者：

Day, J ^{[1
]}

Slawson, M ^{[1
]}

Lugo, RA ^{[1
]}

Wilkins, D ^{[1
]}

机构：

[1] Univ Utah, Ctr Human Toxicol, Salt Lake City, UT 84112 USA

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 2003年 / 27卷 / 07期

关键词：

D O I：

10.1093/jat/27.7.513

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This report describes a sensitive and specific high-performance liquid chromatography (HPLC)-electrospray ionization-tandem mass spectrometry (MS-MS) method for the detection of subnanogram concentrations of fentanyl and its metabolite norfentanyl in human plasma. The assay was based on a liquid-liquid extraction of 0.5 mL of human plasma, with a lower limit of quantitation (LLOQ) of 0.05 ng/mL. Sample extracts were analyzed using a ThermoQuest TSQ® MS-MS interfaced with a Hewlett-Packard series 1100 HPLC and a Phenomenex® (30 × 2.00-mm, 5 μ Luna C18(2)) column. The intra-assay precision and accuracy ranged from 2.1 to 12.5% for both analytes at concentrations of 0.1, 0.5, 1.0, and 10 ng/mL. The interassay accuracy and precision ranged from 7.34 to 10.95%.

引用

页码：513 / 516

页数：4

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