Protein-cross-linked polymeric materials through site-selective bioconjugation

被引：62

作者：

Esser-Kahn, Aaron P. ^{[1
,2
]}

Francis, Matthew B. ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Lawrence Berkeley Natl Lab, Div Mat Sci, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 20期

关键词：

bioconjugation; gels; hybrid materials; polymers; proteins;

D O I：

10.1002/anie.200705564

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Creating a greener future: A strategy for creating hybrid materials of polymers and proteins relies on orthogonal reactions to activate the N and C termini of a protein concurrently. The protein is then used to cross-link polymer chains and create a hydrogel (see picture). A model hybrid hydrogel containing enhanced green fluorescent protein is both biodegradable and responsive to changes in pH and temperature. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3751 / 3754

页数：4

共 29 条

[21] Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy [J].

Patterson, GH ;

Knobel, SM ;

Sharif, WD ;

Kain, SR ;

Piston, DW .

BIOPHYSICAL JOURNAL, 1997, 73 (05) :2782-2790

[22] Dual-surface modification of the tobacco mosaic virus [J].

Schlick, TL ;

Ding, ZB ;

Kovacs, EW ;

Francis, MB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (11) :3718-3723

[23] Maturation efficiency, trypsin sensitivity, and optical properties of Arg96, Glu222, and Gly67 variants of green fluorescent protein [J].

Sniegowski, JA ;

Phail, ME ;

Wachter, RM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 332 (03) :657-663

[24] Comparative studies on the structure and stability of fluorescent proteins EGFP, zFP506, mRFP1, "dimer2", and DsRed1 [J].

Stepanenko, OV ;

Verkhusha, VV ;

Kazakov, VI ;

Shavlovsky, MM ;

Kuznetsova, IM ;

Uversky, VN ;

Turoverov, KK .

BIOCHEMISTRY, 2004, 43 (47) :14913-14923

[25] Tyrosine-selective protein alkylation using π-allylpalladium complexes [J].

Tilley, SD ;

Francis, MB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (04) :1080-1081

[26] The green fluorescent protein [J].

Tsien, RY .

ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :509-544

[27] Hybrid hydrogels assembled from synthetic polymers and coiled-coil protein domains [J].

Wang, C ;

Stewart, RJ ;

Kopecek, J .

NATURE, 1999, 397 (6718) :417-420

[28] REVERSIBLE DENATURATION OF AEQUOREA GREEN-FLUORESCENT PROTEIN - PHYSICAL SEPARATION AND CHARACTERIZATION OF THE RENATURED PROTEIN [J].

WARD, WW ;

BOKMAN, SH .

BIOCHEMISTRY, 1982, 21 (19) :4535-4540

[29] Growth factor mediated assembly of cell receptor-responsive hydrogels [J].

Yamaguchi, Nori ;

Zhang, Le ;

Chae, Byeong-Seok ;

Palla, Chandra S. ;

Furst, Eric M. ;

Kiick, Kristi L. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (11) :3040-+

← 1 2 3 →