Novel mass tags for single nucleotide polymorphism detection

被引：12

作者：

Birikh, Koara R. ^{[1
]}

Korshun, Vladimir A. ^{[1
]}

Bernad, Pablo L. ^{[3
]}

Malakhov, Andrei D. ^{[1
,3
]}

Milner, Natalie ^{[3
]}

Khan, Safraz ^{[3
]}

Southern, Edwin M. ^{[2
]}

Shchepinov, Mikhail S. ^{[3
]}

机构：

[1] RAS, Shemyakin Ovchinikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England

[3] Tridend Technol, Div Oxford Gene Technol Ltd, Oxford OX5 1PF, England

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 07期

关键词：

D O I：

10.1021/ac071291y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method suitable for single nucleotide polymorphism detection and other applications based on oligonucleotide probe extension has been developed. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption/ionization of the cleavable labels. A new family of sulfur-linked laser-cleavable trityl labels with vastly improved flying abilities is implemented in this study. Corresponding reagents compatible with automated oligonucleotide synthesis are presented. Utility of this method for SNP genotyping is demonstrated.

引用

页码：2342 / 2350

页数：9

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